Alkaline phosphatase (ALP) isoenzyme electrophoresis (ALPIE)
Requested in order to determine the source of elevated ALP if not clear. May be of use for possible cases of benign transient hyperphosphatasia of infancy and childhood. Samples are referred to York for ALP isoenzyme electrophoresis. The sample will only be referred for analysis if ALP > 150 iU/L. Requests for isoenzyme analysis with borderline high ALP values may be cancelled at the discretion of the Duty Biochemist. If GGT is raised, this strongly suggests a liver source for ALP and the sample may not be referred for isoenzyme analysis.
| Tube | 01 (SE) - Serum Gel |
|---|---|
| ID | Tests&Tubes0147 |
| Additional Information | When ALP is within the reference range, liver and bone fractions are present in approximately equal proportions. Elevated bone ALP may be due to vitamin D deficiency, hyperparathyroidism, Paget's disease, healing fractures or malignancy. Elevated liver ALP indicates cholestasis (various causes). Increased intestinal ALP can occur in non-fasting healthy individuals with blood group O or B, in diabetes mellitus and a variety of other conditions. Increased placental ALP is normal in pregnancy. |
| Turn Around | Turnaround time stated by York: 2 weeks |
| Send to | Blood Sciences LGI (Test referred to: York Hospital Biochemsitry lab.) |
| Contact | For further details please contact Leeds Pathology customer services: leedsth-tr.pathologycustomerservice@nhs.net. |
| Advice | Full information on all referred tests can be found on the referred tests information database, EQMS reference BSF2REC17003. |
| Collection Con | Instructions to lab: Centrifuge sample, ensure routine tests on the sample are completed. Store in the fridge until dispatch to York (if at SJUH, internal transfer to LGI). Minimum volume: 0.5 mL serum |
| Sample REQ | Blood |
| Ref. Range Notes | Interpretation will be provided on report. |
| Telepath | LGI superset LAPIE; SJUH superset JAPIE. |
