Vitamin B6 (pyridoxal, PLP)
Test not routinely available without discussion with the Duty Biochemist (x26922 option 2). Vitamin B6 is a water-soluble vitamin and is part of the vitamin B complex group. Pyridoxal phosphate is the active form and is a cofactor in many reactions of amino acid metabolism, including transamination, deamination, and decarboxylation. It is also necessary for the enzymatic reaction governing the release of glucose from glycogen.
| Tube | 06 (HP) - Heparin No Gel |
|---|---|
| ID | Tests&Tubes0257 |
| Additional Information | Please contact the lab (x26922 option 2) to discuss before sending the sample to ensure correct processing.. |
| Turn Around | Turnaround time stated by Glasgow Royal Infirmary: 2 weeks. |
| Send to | Blood Sciences LGI (Test referred to: Glasgow Royal Infirmary) |
| Contact | For further details please contact Leeds Pathology customer services: leedsth-tr.pathologycustomerservice@nhs.net. |
| Collection Con | For vitamin B6 status, lithium heparin (no gel) or EDTA tube required. Gel tubes are not suitable. Fasting sample preferred, or 8h post oral supplementation or TPN. For ?hypophosphatasia, fasting EDTA sample required. PROTECT FROM LIGHT (e.g. wrap in foil). Instructions to lab: for vitamin B6, if sample can be sent so as to arrive in Glasgow within 72h of collection, store and send whole blood (ambient), minimum 1 mL. Otherwise, centrifuge, remove plasma and buffy coat, and freeze packed red cells, minimum 400 uL. When booking in, please note whether sample was protected from light in specimen comment. For the diagnosis of hypophosphatasia, EDTA plasma is required, minimum 600 uL. Separate and freeze plasma. Write ‘plasma PLP/PA ratio’ on request form. Light sensitive. |
| Sample REQ | Blood |
| Ref. Range Notes | red cell PLP: 250 - 680 pmol PLP/g Hb. |
| Units | pmol PLP/g Hb |
| Telepath | LGI superset LVITB6. SJUH superset JVITB6. |
