***EMA BINDING is no longer available at LTHT pathology. SAMPLES ARE SENT TO MANCHESTER ROYAL IMMUNOLOGY LAB***
For the diagnosis of hereditary spherocytosis. Eosin 5-Maleimide is a fluorescent dye which binds covalently to band 3 protein in the red cell membrane. Analysis by flow cytometry measures the fluorescence intensity of labelled intact red cells. Indication for requesting: Investigation of a suspected case of hereditary spherocytosis where the FBC result and morphology are indicative, and other causes of haemolysis have been ruled out.
Tube
03 (EB) – EDTA Blood
ID
Tests&Tubes0182
Additional Information
Examination of a blood film for the presence of spherocytes along with a clinical history is important in the evaluation of a reduced EMA binding test result. The presence of spherocytes and a reduced Eosin 5-Maleimide binding test result is suggestive of a diagnosis of hereditary spherocytosis. In some patients the test may not prove to be diagnostic and a diagnosis can only be made using other methods. The sample should be less than 24h old for testing. The patients should not have received a transfusion in the last 3 months.
Turn Around
Turnaround time stated by Manchester Royal Infirmary: 4 days.
Send to
Blood Sciences LGI
(Test referred to: Manchester Royal Infirmary).
Contact
For further details please contact Leeds Pathology customer services: [email protected].
Advice
Full sendaway test details logged internally on the referred test database [BSHC-REC-323].
Collection Con
Minimum Volume – 1 mL EDTA whole blood. Requests MUST be discussed in advance of sample collection and will only be accepted from Clinical Haematology. Please email [email protected]. Samples must be received in the lab by mid-day, Monday – Thursday in order to dispatch to Manchester in time. **Instructions to lab: Do not centrifuge. If sample arrives at SJUH, book in, taxi sample to LGI, notify CSR referrals team. If at LGI, book in, pass sample to referrals section. The sample should reach Manchester within 24h of collection.
Frequency
Sample REQ
Blood.
Ref. Range Notes
Interpretation will be provided on the report.