
Anti-nuclear antibody (ANA) testing can be used for the assessment of systemic autoimmune disease/ connective tissue disease. Indicated in Systemic autoimmune disease/connective tissue disease. The presence of ANA occurs in a number of autoimmune conditions eg SLE, Systemic sclerosis, Sjogren’s syndrome, dermato/polymyositis, and mixed connective tissue disease. Different autoantibody specificities are associated with different connective tissue diseases. Results from the ANA screen should always be interpreted in the context of relevant clinical signs and symptoms.
Tube
Serum Gel
ID
12751
Availability
Weekdays
Additional Information
A multiplex flow immunoassay using the Bioplex 2200 has replaced indirect immunofluorescence and anti-DNA and ENA Ab ELISA methods for the detection of ANA. NEW positive results will be confirmed using Indirect Immunofluorescence. The multiplex ANA screen can detect the presence of 12 different ANA components: dsDNA, Ro60, Ro52, La, Sm, SmRNP, RNP-68, CENP-B (centromere), Scl-70, Jo-1, chromatin and ribosomal P Ab. Detection of one or more of these autoantibody specificities can be useful in the diagnosis of systemic autoimmune/connective tissue diseases. If ANA by indirect immune fluorescence is required in addition to screening on the Bioplex analyser, you must first discuss this with the Consultant Immunologists.
Turn Around
7 days
Send to
Leeds General Infirmary
Clinical Immunology
Old Medical School
Great George Street
Leeds
LS1 3EX
Collection Con
No restrictions on collection conditions HOWEVER please note that this test is not appropriate for pleural fluid samples.
Frequency
Daily
Sample REQ
Blood
Ref. Range (Male)
See reference range note
Ref. Range (Female)
See reference range note
Ref. Range (Paed)
See reference range note
Ref. Range Notes
ANA screen: Positive or negative. The ANA screen will be reported as positive if the patient is positive for one or more of the 12 specific autoantigens. Positive anti-DNA Ab = 10IU/mL or above. Positive anti-ENA Ab – 1AI or above, except anti-La and -Scl-70 = 2.5AI or above. ANA by IIF is reported as POS/WEAK/NEG
Units
dsDNA Ab IU/mL ENA Ab AI (antibody Index)
IP Acute TAT
Contact Laboratory
IP Routine TAT
7 days
GP Acute TAT
Contact Laboratory
GP Routine TAT
7 days