Important notice:
If a CSF sample requires urgent processing, the laboratory must be informed. Telephone on ext. 69403 (0113 206 9403 for non LTHT NPP users) during working hours (8.30am – 5.00pm) or mobile 07880 786 778 during out-of-hours (5.00pm – 8.30am).
Investigation of suspected bacterial meningitis or subarachnoid bleed. Suspected bacterial meningitis should be confirmed by lumbar puncture. A high white cell count along with positive gram stain would support diagnosis. For suspected SAH, send sample 1 and 3 to compare counts. Containers must be clearly labelled. Where possible send separate tubes for each test requested.
Tube
Sterile universal.
ID
3619.
Availability
Processing 24/7 Positive results released to server 24/7. Please contact laboratory prior to sending.
Additional Information
Pre antibiotic CSF will give better culture yields but this does not imply to withhold antibiotic therapy.
Send to
Microbiology Department, Centre for Laboratory Medicine, St James’s University Hospital, Beckett Street, Leeds, LS9 7TF.
Collection Con
Observe aseptic sampling technique for performing lumbar puncture and decanting sample into sterile universal containers. Transport the sample to the laboratory as quickly as possible. Note: The recovery of anaerobes is compromised if the transport time exceeds 3 hours. The time between collection of CSF samples to microscopy and culture should occur within a maximum of 2 hours. Cells disintegrate and a delay may produce a cell count that does not reflect the clinical situation of the patient.
Sample REQ
Min vol 0.5 ml. If further tests are required contact ext 69461 during working hours.
IP Routine TAT
Cell Count – within 2 hours if laboratory is notified prior to arrival. Standard culture -5 days. Neuro ward culture -7 days.
GP Routine TAT
Cell Count – within 2 hours if laboratory is notified prior to arrival. Standard culture -5 days. Neuro ward culture -7 days.