Investigation of suspected Clostridium difficile infection in at risk populations. GDH (Glutamate Dehydrogenase) testing is by ELISA. Cytotoxin production is tested by ELISA. Confirmation of toxin production indicates active disease. At LTHT a further PCR test is carried out on samples that are GDH positive but toxin negative to detect the presence of the toxin gene and therefore guide IC isolation procedures.
Container / Tube
Brown top with spoon.
Collection Conditions
A minimum 2g of faecal sample is required. Samples must be received by the laboratory within 72 hours of been taken.
Availability
Monday – Sunday, 8:30am – 5:00pm.
Send to
Microbiology Department, Centre for Laboratory Medicine, St James’s University Hospital, Beckett Street, Leeds, LS9 7TF.
Turnaround Time
Screening test within 24 hours. Confirmation of toxin production 5 days.
Additional Information
If patient has significant risk factors e.g., Antibiotic exposure or recent hospitalisation please state clearly on request.
Repeated testing of negatives is not indicated within 7 days. Repeated testing of positives is not indicated within 14 days. If repeat testing is indicated sooner, please contact Microbiology (Ext 23962).
ID
3610