Investigation of suspected Clostridium
difficile infection in at risk populations.
GDH (Glutamate Dehydrogenase) testing is by ELISA
Cytotoxin production is tested by tissue culture. Confirmation of toxin production indicates active disease.
At LTHT a further PCR test is carried out on samples that are GDH positive but toxin negative to detect the presence of the toxin gene and therefore guide IC isolation procedures.
Tube
Brown top with spoon
ID
3610
Availability
Mon to Sun. 8.30am to 5pm.
Additional Information
If patient has significant risk factors eg. Antibiotic
exposure or recent.
hospitalisation please state clearly on request.
Turn Around
Screening test within 24 hours. Confirmation of toxin production 5 days.
Send to
LGI Microbiology Department.
Collection Con
Samples must be received by the laboratory within 72 hours of been taken.
Frequency
Repeated testing of negatives is not indicated within 7 days. Repeated testing of positives is not indicated within 14 days. If repeat testing is indicated sooner, please contact Microbiology (Ext23962).
Sample REQ
A minimum 2g of faecal sample is required.
IP Routine TAT
Screening test within 24 hours confirmation of toxin production 5 days.
GP Routine TAT
Screening test within 24 hours confirmation of toxin production 5 days.