***EMA BINDING is no longer available at LTHT pathology. SAMPLES ARE SENT TO MANCHESTER ROYAL IMMUNOLOGY LAB***
For the diagnosis of hereditary spherocytosis. Eosin 5-Maleimide is a fluorescent dye which binds covalently to band 3 protein in the red cell membrane. Analysis by flow cytometry measures the fluorescence intensity of labelled intact red cells. Indication for requesting: Investigation of a suspected case of hereditary spherocytosis where the FBC result and morphology are indicative, and other causes of haemolysis have been ruled out.
Tube
03 (EB) – EDTA Blood
ID
Tests&Tubes0182
Additional Information
Examination of a blood film for the presence of spherocytes along with a clinical history is important in the evaluation of a reduced EMA binding test result. The presence of spherocytes and a reduced Eosin 5-Maleimide binding test result is suggestive of a diagnosis of hereditary spherocytosis. In some patients the test may not prove to be diagnostic and a diagnosis can only be made using other methods. The sample should be less than 24h old for testing. The patients should not have received a transfusion in the last 3 months.
Turn Around
Turnaround time stated by Manchester Royal Infirmary: 4 days.
Send to
Blood Sciences LGI
(Test referred to: Manchester Royal Infirmary).
Contact
For further details please contact Leeds Pathology customer services: [email protected].
Advice
Full sendaway test details logged internally on the referred test database [BSF2REC17003].
Collection Con
Minimum Volume – 1 mL EDTA whole blood.
Requests MUST be discussed in advance of sample collection. Please email [email protected], [email protected] & [email protected]t. Samples must be received in the lab by mid-day, Monday – Thursday in order to dispatch to Manchester in time. **Instructions to lab: Do not centrifuge. If sample arrives at SJUH, book in, taxi sample to LGI, notify Eleanor McLaughlan / Jennifer Spencer / Kevin Stuart. If at LGI, book in, pass sample to referrals section and notify Eleanor McLaughlan / Jennifer Spencer / Kevin Stuart. The sample should reach Manchester within 24h of collection.
Frequency
Sample REQ
Blood.
Ref. Range Notes
Interpretation will be provided on the report.