3-hydroxybutyrate is one of three ketone bodies generated from free fatty acids via ketogenesis. Both free fatty acids and beta-hydroxybutyrate are analysed on the sample. Clinical indications for request: ? Hyperinsulinism, ? Fat oxidation defects (e.g. MCADD), ? Ketolytic defects (e.g. beta ketothiolase deficiency), ketoacidotic states (e.g. alcoholic).
Tube
07 (FP) – Fluoride Oxalate Plasma
ID
Tests&Tubes0189
Additional information
Please collect into a fluoride oxalate (grey-topped) tube. Serum samples can also be accepted.
For the investigation of hyperinsulinism or fat oxidation defect, the plasma glucose should be <2.5 mmol/L at the time of sampling for analysis to be valid.
For ketolytic defect investigations, the test should be performed whatever the glucose.
For hyperinsulinism, fat oxidation defects and ketolytic defects refer to Paediatric Biochemist at SJUH (ext 64256).
Turn around
Turnaround time stated by Edinburgh Royal Infirmary: 5 days
Send to
Blood Sciences, Centre for Laboratory Medicine, St James’s University Hospital, Beckett Street, Leeds, LS97TF (Test referred to: Edinburgh Royal Infirmary)
Contact
For further details please contact Leeds Pathology customer services: [email protected].
Advice
Full information on all referred tests can be found on the referred tests information database, EQMS reference BSF2REC17003.
Collection Con
Instructions to lab: Centrifuge, separate and store plasma in the fridge prior to sending. If sample postage is expected to be delayed, separated samples should be frozen before sending. Minimum volume: 0.5 mL plasma/ serum.
Sample REQ
Blood
Sample Storage
Separated plasma samples are stable at room temperature for at least 4 days. After separation, store samples in the fridge before sending to Edinburgh via first class post at ambient temperature. If sample postage will be delayed (for example over the weekend), samples should be frozen prior to sending via first class post at ambient temperature.
Ref Range Notes
FFA: 0.1 – 0.6 mmol/L. BOHB: < 0.4 mmol/L. Will be reported with an interpretive comment.
Units
mmol/L