Anaphylactic reactions (IgE dependant or non-IgE dependant) are mediated by the release of vasoactive mediators, including tryptase, from mast cells and circulating basophils. Serial measurements of Tryptase are made to determine if a mast cell-mediated reaction to the anaesthetic is evident and the investigation of anaphylactic reactions requires 3 serial samples (see collection conditions).
Tube
Serum Gel
ID
12799
Availability
Weekdays
Additional Information
Tryptase levels usually peak within an hour and has an in vivo half-life of 2.5 hours, returning to normal within 12 to 24 hours after release.
Plasma tryptase can be elevated in systemic mastocytosis. Here levels tend to be constantly raised. Mastocytosis is a rare disorder. The presence of systemic mast cell degranulation is evidenced by an increase in serum tryptase however tryptase levels are persistently elevated in mastocytosis therefore random measurements are difficult to interpret.
Please contact the Immunology Lab 0113 39 23414 (between 08:30am and 5:00pm) to advise them a sample has been sent. If sending out of hours, please contact the main lab on 0113 39 26745 to advise them a sample has been sent.
Please contact the laboratory for interpretive advice on post mortem samples.
Turn Around
7 days
Send to
Leeds General Infirmary
Clinical Immunology
Old Medical School
Great George Street
Leeds
LS1 3EX
Collection Con
Sample 1: As soon as possible after, or within one hour or, onset of reation.
Sample 2: 3-6 h post-reation (peak reaction time)
Sample 3: 24h post-reation (baseline). Samples should be taken as near as possible to these times after the suspected ana
Please contact the Immunology Lab 0113 39 23414 (between 08:30am and 5:00pm) to advise them a sample has been sent. If sending out of hours, please contact the main lab on 0113 39 26745 to advise them a sample has been sent.
Instructions for Lab staff: As soon as the sample arrives in reception centrifuge and store serum in the freezer.
Frequency
Daily
Sample REQ
Blood
Units
ng/mL
IP Acute TAT
Contact Laboratory
IP Routine TAT
7 days
GP Acute TAT
Contact Laboratory
GP Routine TAT
7 days