Anti-nuclear antibodies (ANA) Screen -Bioplex Method
Anti-nuclear antibody (ANA) testing can be used for the assessment of systemic autoimmune disease/ connective tissue disease. Indicated in Systemic autoimmune disease/connective tissue disease. The presence of ANA occurs in a number of autoimmune conditions eg SLE, Systemic sclerosis, Sjogren's syndrome, dermato/polymyositis, and mixed connective tissue disease. Different autoantibody specificities are associated with different connective tissue diseases. Results from the ANA screen should always be interpreted in the context of relevant clinical signs and symptoms. Please see pathology web pages for further information http://www.pathology.leedsth.nhs.uk/pathology/ClinicalInfo/Immunology/Autoimmunedisease.aspx
| Tube | Serum Gel |
|---|---|
| ID | 12751 |
| Availability | Weekdays |
| Additional Information | A multiplex flow immunoassay using the Bioplex 2200 has replaced indirect immunofluorescence and anti-DNA and ENA Ab ELISA methods for the detection of ANA. NEW positive results will be confirmed using Indirect Immunofluorescence. The multiplex ANA screen can detect the presence of 12 different ANA components: dsDNA, Ro60, Ro52, La, Sm, SmRNP, RNP-68, CENP-B (centromere), Scl-70, Jo-1, chromatin and ribosomal P Ab. Detection of one or more of these autoantibody specificities can be useful in the diagnosis of systemic autoimmune/connective tissue diseases. If ANA by indirect immune fluorescence is required in addition to screening on the Bioplex analyser, you must first discuss this with the Consultant Immunologists. |
| Turn Around | 7 days |
| Send to | Leeds General Infirmary Clinical Immunology Old Medical School Great George Street Leeds LS1 3EX |
| Advice | http://www.pathology.leedsth.nhs.uk/pathology/ClinicalInfo/Immunology/Autoimmunedisease.aspx |
| Collection Con | No restrictions on collection conditions HOWEVER please note that this test is not appropriate for pleural fluid samples. |
| Frequency | Daily |
| Sample REQ | Blood |
| Ref. Range (Male) | See reference range note |
| Ref. Range (Female) | See reference range note |
| Ref. Range (Paed) | See reference range note |
| Ref. Range Notes | ANA screen: Positive or negative. The ANA screen will be reported as positive if the patient is positive for one or more of the 12 specific autoantigens. Positive anti-DNA Ab = 10IU/mL or above. Positive anti-ENA Ab - 1AI or above, except anti-La and -Scl-70 = 2.5AI or above. ANA by IIF is reported as POS/WEAK/NEG |
| Units | dsDNA Ab IU/mL ENA Ab AI (antibody Index) |
| IP Acute TAT | - Contact Laboratory |
| IP Routine TAT | 7 days |
| GP Acute TAT | - Contact Laboratory |
| GP Routine TAT | 7 days |
| Telepath | ANAS1L |
| ICE Codes | IANA |
