The Leeds Teaching Hospitals NHS Trust

Clostridium difficile GDH & toxin testing

Clostridium difficile GDH & toxin testing

Investigation of suspected Clostridium
difficile infection in at risk populations.

GDH (Glutamate Dehydrogenase) testing is by ELISA
Cytotoxin production is tested by tissue culture. Confirmation of toxin production indicates active disease.

At LTHT a further PCR test is carried out on samples that are GDH positive but toxin negative to detect the presence of the toxin gene and therefore guide IC isolation procedures.

Tube Brown top with spoon
ID 3610
Availability Mon- Sun 8.30 - 5pm
Additional Information If patient has significant risk factors eg. Antibiotic
exposure or recent
hospitalisation please state clearly on request.
Turn Around Screening test within 24 hours. Confirmation of toxin production 5 days
Send to LGI Microbiology Department
Collection Con Samples must be received by the laboratory within 72 hours of been taken.
Frequency Repeated testing of negatives is not indicated within 7 days. Repeated testing of positives is not indicated within 14 days. If repeat testing is indicated sooner, please contact Microbiology(Ext23962
Sample REQ A minimum 2g of faecal sample is required.
IP Routine TAT Screening test within 24 hours confirmation of toxin production 5 days
GP Routine TAT Screening test within 24 hours confirmation of toxin production 5 days