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Glucose 6-Phosphate Dehydrogenase

Glucose 6-Phosphate Dehydrogenase (G6PD) is a metabolic enzyme found in red blood cells. It plays a role in the pentose phosphate pathway protecting the red cell from oxidative damage. G6PD deficiency is sex linked and common amongst certain ethnic groups. G6PD deficiency can cause a haemolytic anaemia when a patient is exposed to oxidative stress.

Tube	03 (EB) - EDTA Blood
ID	Tests&Tubes0060
Additional Information	All non-LTHT requests now require a referral form to be completed, which can be found at https://www.leedsth.nhs.uk/a-z-of-services/pathology/blood-sciences/useful-information-and-links/. Sample Requirements:Please send 1 x Fresh EDTA patient sample (3ml minimum volume) along with a travel control. Travel control = ANONYMYSED EDTA (1ml minimum volume) sample clearly labelled as travel control. This sample must be suspected to have normal G6PD activity and collected under similar circumstances to the test sample [FBC & Reticulocytes must be normal]. A travel control is also required to accompany the sample.
Turn Around	A&E: 120 hours Day Case: 120 hours In-Patient: 120 hours Out-Patient: 120 hours GP: 120 hours
Send to	Blood Sciences SJUH
Contact	For further details please contact Leeds Pathology customer services: leedsth-tr.pathologycustomerservice@nhs.net.
Collection Con	Reticulocytes have higher G6PD levels than mature red cells. It is recommended that assays not be performed after a severe haemolytic crisis, since G6PD levels may appear falsely elevated. Instructions for lab: LTHT samples book in as G6PD1J and send sample for FBC and retic, sample is then stored at 2-8C until analysis. Referral samples (non-LTHT) book in as G6PR1J. Label referral form/FBC data and travel control with the eye-readable barcodes and place in the G6PD tray in the cold room until analysis. Referral samples that do not arrive with a travel control should be rejected and not processed.
Sample REQ	EDTA blood 3ml
Ref. Range Notes	Blood Sciences Refernce ranges available through the useful information and links page:https://www.leedsth.nhs.uk/a-z-of-services/pathology/blood-sciences/useful-information-and-links/
Telepath	G6PD1J (LTHT request) and G6PR1J (non-LTHT request)
ICE Codes	G6PD1J