The Leeds Teaching Hospitals NHS Trust

AAFB Microscopy and Culture

AAFB Microscopy and Culture

Auramine microscopy and continuous monitored incubation for culture of AAFB.
Indicated in the investigation of:
• Pulmonary tuberculosis
• Extra pulmonary tuberculosis can present as ""cold"" abscesses in almost all sites.
• Lymphadenitis
• Bone and joint, including spinal, tuberculosis
• Renal mycobacterial disease
Further identification and susceptibility testing are provided following referral to Birmingham PHL laboratory

Tube Sputum potWhite universal for other samples
ID 3613
Availability Mon-Fri8.30-5pm
Additional Information Sample type is dependent on the suspected site of infection.
Full clinical details are essential to providing correct test conditions
Respiratory:BAL, ET secretons, pleural aspirate, tissue biopsy
Renal: Early morning urine sample
TB Meningitis: CSF
Extra pulmonary: dependent on site of disease, include lymph nodes, tissue, pus, gastric biopsies.
If in doubt contact laboratory.
Tuberculosis is a notifiable disease.
Turn Around Microscopy for AAFB available within 24 hours for samples received from Mon-Fri before 12pm. TAT for samples received weekends and bank holidays will be 3 days

Culture 60 days. TAT may be increased if prolonged culture is indicated

Send to LGI Microbiology Department
Frequency 3 early morning samples on consecutive days for sputum and urine samples
IP Routine TAT Microscopy for AAFB available within 24 hours for samples received from Mon-Fri before 12pm. TAT for samples received weekends and bank holidays will be 3 daysCulture 60 days. TAT may be increased
GP Routine TAT Microscopy for AAFB available within 24 hours for samples received from Mon-Fri before 12pm. TAT for samples received weekends and bank holidays will be 3 daysCulture 60 days. TAT may be increased