The Leeds Teaching Hospitals NHS Trust

Cerebro-spinal fluid (Microscopy and Culture)

Cerebro-spinal fluid (Microscopy and Culture)

Investigation of suspected bacterial meningitis or subarachnoid bleed.
Suspected bacterial meningitis should be confirmed by lumbar puncture. A high white cell count along with positive gram stain would support diagnosis.
For suspected SAH send sample 1 & 3 to compare counts.
Containers must be clearly labelled. Where possible send separate tubes for each test requested.

Tube Sterile universal
ID 3619
Availability Processing 24/7Positive results released to server 24/7Please contact laboratory prior to sending.
Additional Information Pre antibiotic CSF will give better culture yields but this does not imply to withhold antibiotic therapy.
Turn Around Cell Count - within 1 hour if laboratory is notified prior to arrivalStandard culture -5 daysNeuro ward culture -7 days
Send to LGI Microbiology Department
Collection Con Observe aseptic sampling technique for performing lumbar puncture and decanting sample into sterile universal containers. Transport the sample to the laboratory as quickly as possible. Note: The recovery of anaerobes is compromised if the transport time exceeds 3 hours.
Sample REQ Min vol 0.5ml. If further tests required contact
IP Routine TAT Cell Count - within 1 hour if laboratory is notified prior to arrivalStandard culture -5 daysNeuro ward culture -7 days
GP Routine TAT Cell Count - within 1 hour if laboratory is notified prior to arrivalStandard culture -5 daysNeuro ward culture -7 days